J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 313689 |
313689
|
JB-STOCK
|
shmiR line
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w; pW20>nbr_sh2[attp40]/CyO ; ;
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attP40 landing site; top primer: ctagcAGTCAGCAGCAACATCAAGACGAATAGTTATATTCAAGCATATTCGTCTTGATGTTGCTGCTGGCg
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313689
|
Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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attP40 landing site; top primer: ctagcAGTCAGCAGCAACATCAAGACGAATAGTTATATTCAAGCATATTCGTCTTGATGTTGCTGCTGGCg
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| 313690 |
313690
|
JB-STOCK
|
shmiR line
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w; If/CyO; pW20>nbr_sh2[attp2]/TM3,Ser;
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attP2 landing site; top primer: ctagcAGTCAGCAGCAACATCAAGACGAATAGTTATATTCAAGCATATTCGTCTTGATGTTGCTGCTGGCg
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313690
|
Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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attP2 landing site; top primer: ctagcAGTCAGCAGCAACATCAAGACGAATAGTTATATTCAAGCATATTCGTCTTGATGTTGCTGCTGGCg
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| 313693 |
313693
|
JB-STOCK
|
shmiR line
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w; pW22>zuc_sh1[attp40]/CyO; Sb/TM3,Ser;
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attP40 landing site, same sequence as TRIP #GL00111, but the knockdown is weaker; top primer: ctagcAGTCACGAACTTGATGCACAACAATAGTTATATTCAAGCATATTGTTGTGCATCAAGTTCGTGGCg
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313693
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Hayashi & Schnabl, Nature, 2016
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zucchini
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empty
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attP40 landing site, same sequence as TRIP #GL00111, but the knockdown is weaker; top primer: ctagcAGTCACGAACTTGATGCACAACAATAGTTATATTCAAGCATATTGTTGTGCATCAAGTTCGTGGCg
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| 313694 |
313694
|
JB-STOCK
|
shmiR line
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w; ; pw20>zuc_sh1+white_sh[attP2]/TM3, Sb;
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attP2 landing site, control construct for double depletion, 1st zuc_shmiR sequence is identical to GL00111, 1st top primer(zucchini): CTAGCAGTTTGTTGTGCATCAAGTTCGTGTAGTTATATTCAAGCATACACGAACTTGATGCACAACAAGCG, 2nd top primer(white): ccggtAGTAACAAGATATGACCTATAGAATAGTTATATTCAAGCATATTCTATAGGTCATATCTTGTTGCgcatg
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313694
|
Hayashi & Schnabl, Nature, 2016
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zucchini, white
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empty
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attP2 landing site, control construct for double depletion, 1st zuc_shmiR sequence is identical to GL00111, 1st top primer(zucchini): CTAGCAGTTTGTTGTGCATCAAGTTCGTGTAGTTATATTCAAGCATACACGAACTTGATGCACAACAAGCG, 2nd top primer(white): ccggtAGTAACAAGATATGACCTATAGAATAGTTATATTCAAGCATATTCTATAGGTCATATCTTGTTGCgcatg
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| 313695 |
313695
|
JB-STOCK
|
shmiR line
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w; If/CyO; pW20>zuc_sh+nbr_sh[attP2]/TM3,Ser;
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attP2 landing site, 1st zuc_shmiR sequence is identical to GL00111, 2nd nibbler_shmiR sequence is identical to nbr_sh2, 1st top primer(zucchini): CTAGCAGTTTGTTGTGCATCAAGTTCGTGTAGTTATATTCAAGCATACACGAACTTGATGCACAACAAGCG, 2nd top primer(nibbler): ccggtAGTATGGTCAGTGATCTCAGTGTATAGTTATATTCAAGCATATACACTGAGATCACTGACCATGCgcatg
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313695
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Hayashi & Schnabl, Nature, 2016
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zucchini, nibbler
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empty
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attP2 landing site, 1st zuc_shmiR sequence is identical to GL00111, 2nd nibbler_shmiR sequence is identical to nbr_sh2, 1st top primer(zucchini): CTAGCAGTTTGTTGTGCATCAAGTTCGTGTAGTTATATTCAAGCATACACGAACTTGATGCACAACAAGCG, 2nd top primer(nibbler): ccggtAGTATGGTCAGTGATCTCAGTGTATAGTTATATTCAAGCATATACACTGAGATCACTGACCATGCgcatg
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| 313700 |
313700
|
JB-STOCK
|
CRISPR line
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w; nibbler[Cas9 FS allele]/CyO-GFP; ;
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313700
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Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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| 313703 |
313703
|
JB-STOCK
|
CRISPR line
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w; FLAG-nibbler[wt]/CyO; ;
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endogenous Nibbler allele with N-terminal 3xFLAG tag
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313703
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Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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endogenous Nibbler allele with N-terminal 3xFLAG tag
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| 313705 |
313705
|
JB-STOCK
|
CRISPR line
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w; papi[1-5-2]; ;
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papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA
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313705
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Hayashi & Schnabl, Nature, 2016
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papi
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empty
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papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA
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| 313706 |
313706
|
JB-STOCK
|
CRISPR line
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w; papi[4-1-1]/CyO; ;
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papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG
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313706
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Hayashi & Schnabl, Nature, 2016
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papi
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empty
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papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG
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| 313713 |
313713
|
JB-STOCK
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Pacman recombineering
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w; If/CyO; 3xFLAG_V5_Precission_GFP_Nbr[attP2]/TM3, Ser;
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Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807
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313713
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Hayashi & Schnabl, Nature, 2016
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nibbler
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empty
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Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807
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| 313714 |
313714
|
JB-STOCK
|
Pacman recombineering
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Cog-GAL4; NGT-GAL4; nos-GAL4, Zuc_GFP_Precission_V5_3xFLAG[attP2]/TM3, Ser;
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313714
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Hayashi & Schnabl, Nature, 2016
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zucchini
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empty
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| 313715 |
313715
|
JB-STOCK
|
Pacman recombineering
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w; Papi_GFP_Precission_V5_3xFLAG[attP40]/CyO; Sb/TM3, Ser;
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Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB569; Glycerol #735
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313715
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Hayashi & Schnabl, Nature, 2016
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papi
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empty
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Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB569; Glycerol #735
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