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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 314062 |
314062
|
JB-STOCK
|
tagged construct
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w; ; CG2678RMCE[3xFLAG-ouibZAD]/TM3, Sb
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Tagged Kipferl construct (RMCE); 3xFLAG tagged ZAD replacement construct inserted into 'LB1-RMCEm31'. Aka: L30. Homozygous females subfertile.
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314062
|
Julius Brennecke
|
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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kipf
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3x
B FLAG Kipf RMCE ZAD anon-84Eb kipf kipferl ouib tag transcript B |
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Tagged Kipferl construct (RMCE); 3xFLAG tagged ZAD replacement construct inserted into 'LB1-RMCEm31'. Aka: L30. Homozygous females subfertile.
|
kipferl
|
RMCE
3x ZAD ouib FLAG tag |
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| 314063 |
314063
|
JB-STOCK
|
tagged construct
|
w; ; CG2678RMCE[?ZnF#4-S161-3xFLAG]/TM3, Sb
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Tagged Kipferl construct (RMCE); 3xFLAG tagged ZnF4 deletion construct inserted into 'LB1-RMCEm31'. Aka: L50. Homozygous females subfertile.
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314063
|
Julius Brennecke
|
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
|
kipf
|
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3x
B FLAG Kipf RMCE S161 ZnF anon-84Eb kipf kipferl tag transcript B |
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Tagged Kipferl construct (RMCE); 3xFLAG tagged ZnF4 deletion construct inserted into 'LB1-RMCEm31'. Aka: L50. Homozygous females subfertile.
|
kipferl
|
tag
3x FLAG S161 ZnF RMCE |
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| 314064 |
314064
|
JB-STOCK
|
tagged construct
|
w; pRhino>3xFLAG/V5/Precission/GFP-Rhino(CSD::GCN4)[attP40], rhi[g3m13]/CyO
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Tagged Rhino construct; Rhino indel –14; Rhino CSD was replaced by the Gcn4 dimerization domain; aka: L03. Homozygous females subfertile.
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314064
|
Julius Brennecke
|
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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rhi
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3x
B CG2678 CG32135 CSD FLAG GCN4 GFP HP1 HP1D HP1d Heterochromatin protein 1 Kipf NXF3 Nuclear export factor 3 Nxf3 Precission RHI RHINO Rhi Rhino V5 anon-84Eb g3m13 heterochromatin |
TGGGCGTCCCCAGG---------------AGCGGTTTTCCGAACGAGAACAACACC
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Tagged Rhino construct; Rhino indel –14; Rhino CSD was replaced by the Gcn4 dimerization domain; aka: L03. Homozygous females subfertile.
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rhino
|
FLAG
V5 tag CSD pRhino Precission g3m13 3x GCN4 GFP |
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| 314065 |
314065
|
JB-STOCK
|
tagged construct
|
w; pRhino>3xFLAG/V5/Precission/GFP-Rhino(art.hinge)[attP40], rhi[g3m13]/CyO
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Tagged Rhino construct; Rhino indel –14; Rhino hinge was replaced by a scrambled amino acid sequence of the same length; aka: L17. Homozygous females subfertile.
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314065
|
Julius Brennecke
|
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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rhi
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3x
B CG2678 CG32135 FLAG GFP HP1 HP1D HP1d Heterochromatin protein 1 Kipf NXF3 Nuclear export factor 3 Nxf3 Precission RHI RHINO Rhi Rhino V5 anon-84Eb heterochromatin protein 1 hp1d kip |
TGGGCGTCCCCAGG---------------AGCGGTTTTCCGAACGAGAACAACACC
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Tagged Rhino construct; Rhino indel –14; Rhino hinge was replaced by a scrambled amino acid sequence of the same length; aka: L17. Homozygous females subfertile.
|
rhino
|
GFP
tag Precission 3x FLAG pRhino V5 |
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| 314066 |
314066
|
JB-STOCK
|
tagged construct
|
w; ; pRhino>3xFLAG/V5/Precission/GFP-Su(var)2-5[attP2]
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Tagged HP1a construct; aka: L48
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314066
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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Su(var)205
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3x
C1A9-nuclear-antigen CBX5 DmHP-1 DmHP1 E(var)29 E(var)29A FLAG GFP HP-1 HP1 HP1-VS HP1? HP1A HP1a HP1alpha Heterochromatic Protein 1 Heterochromatic Protein 1a Heterochromatin Protein 1 Heterochr |
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Tagged HP1a construct; aka: L48
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Suppressor of variegation 205
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Precission
art 3x hinge pRhino V5 g3m13 tag FLAG GFP |
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| 314067 |
314067
|
JB-STOCK
|
tagged construct
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w, 3xFLAG/V5/Precission/GFP-HP1b
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Endogenously tagged HP1b; aka: LB3-f212.
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314067
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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HP1b
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3x
FLAG GFP HP1 HP1B HP1b Heterochromatin Protein 1B Heterochromatin Protein 1b Hp1b Precission V5 hp1b tag |
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Endogenously tagged HP1b; aka: LB3-f212.
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Heterochromatin Protein 1b
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3x
GFP tag FLAG Precission V5 |
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| 314068 |
314068
|
JB-STOCK
|
tagged construct
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w; ; 3xFLAG/V5/Precission/GFP-HP1c
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Endogenously tagged HP1c; aka: LB4-f212.
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314068
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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HP1c
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3x
FLAG GFP HP1 HP1C HP1c Heterochromatin Protein 1c Heterochromatin protein 1c Hp1c Precission V5 heterochromatin protein 1c hp1c tag |
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Endogenously tagged HP1c; aka: LB4-f212.
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Heterochromatin Protein 1c
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FLAG
Precission tag 3x GFP V5 |
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| 314069 |
314069
|
JB-STOCK
|
tagged construct
|
w; peggless>TurboID-linker-vhhGFP-3xHA-NLS[attP40]/CyO
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TurboID biotin ligase fused to GFP nanobody, expressed from eggless regulatory elements. Aka: CY59.
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314069
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Julius Brennecke
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Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)
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GFP
GFP nanobody HA NLS TurboID biotin eggless fusion construct ligase nanobody synthetic construct vhh |
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TurboID biotin ligase fused to GFP nanobody, expressed from eggless regulatory elements. Aka: CY59.
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NLS
ligase nanobody HA GFP eggless vhh fusion construct synthetic construct TurboID GFP nanobody biotin |
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| 310600 |
310600
|
FB-STOCK
|
tagged construct
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w*; me31B[KI.EGFP]
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The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
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310600
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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me31B
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C-terminal EGFP tag
DDX6 Dhh1p/Me31b DmRH3 EGFP ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 maternal expression at 31B me31B me31b |
see publication
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The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
|
maternal expression at 31B
|
knock-in
EGFP C-terminal EGFP tag P-body marker |
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| 310601 |
310601
|
FB-STOCK
|
tagged construct
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w*; me31B[KI.mTomato]
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The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
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310601
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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me31B
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C-terminal mTomato tag
DDX6 Dhh1p/Me31b DmRH3 ME31B Mat31B Maternal expression at 31B Me31B Me31b P-body marker knock-in l(2)k06607 mTomato maternal expression at 31B me31B me31b |
see publication
|
|
The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
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maternal expression at 31B
|
P-body marker
knock-in mTomato C-terminal mTomato tag |
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| 310602 |
310602
|
FB-STOCK
|
tagged construct
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w*; ; rin[KI.mCherry]
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The mCherry sequence was inserted via Crispr/Cas9 in the rin locus, at the N-terminus of the rin coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mCherry. Rin is the Drosophila ortholog of the G3BP1; it is Stress Granule marker positive.
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310602
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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rin
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N-terminal mCherry tag
Next to squid Nts RIN Rasputin Rin Stress Granule marker cg9412 knock-in mCherry rasp rasputin rin |
see publication (Formicola et al., 2021; doi: 10.7554/eLife.65742.)
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The mCherry sequence was inserted via Crispr/Cas9 in the rin locus, at the N-terminus of the rin coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mCherry. Rin is the Drosophila ortholog of the G3BP1; it is Stress Granule marker positive.
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rasputin
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N-terminal mCherry tag
Stress Granule marker mCherry knock-in |
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| 310603 |
310603
|
FB-STOCK
|
tagged construct
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w*; ; Patr-1[KI.mTomato]
|
The mTomato sequence was inserted via Crispr/Cas9 in the Patr-1 locus, at the C-terminus of the Patr-1 coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. Patr-1 is the Drosophila ortholog of PATL1; it can be used as a P-body marker.
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310603
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Florence Besse
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Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
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Patr-1
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BcDNA:LD27979
C-terminal mTomato tag HPat HPat1 Hpat LD27979 P-body marker Part-1 Patr-1 Protein associated with topo II related - 1 anon-WO0118547.282 hpat knock-in l(3)06442 lethal (3) 06442 mTomato patr-1 |
see publication (Formicola et al., 2021; doi: 10.7554/eLife.65742.)
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The mTomato sequence was inserted via Crispr/Cas9 in the Patr-1 locus, at the C-terminus of the Patr-1 coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. Patr-1 is the Drosophila ortholog of PATL1; it can be used as a P-body marker.
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Protein associated with topo II related - 1
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P-body marker
knock-in C-terminal mTomato tag mTomato |
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