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Items 1-12 of 17

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Name VDRC Id Library Sub-Category Genotype Comment Construct Id Publication Provider Scientist Provider Organization Gene Symbol FlyBase gene number Synonyms Sequence RRID Comment Gene Name Keywords Price (net) Add to Cart
310600
310600
FB-STOCK
tagged construct
w*; me31B[KI.EGFP]
The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
310600
Florence Besse
Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
me31B
C-terminal EGFP tag
DDX6
Dhh1p/Me31b
DmRH3
EGFP
ME31B
Mat31B
Maternal expression at 31B
Me31B
Me31b
P-body marker
knock-in
l(2)k06607
maternal expression at 31B
me31B
me31b
see publication
The EGFP sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae EGFP positive. Me31B is the Drosophila ortholog of DDX-6; it is a P-body marker.
maternal expression at 31B
knock-in
EGFP
C-terminal EGFP tag
P-body marker
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310601
310601
FB-STOCK
tagged construct
w*; me31B[KI.mTomato]
The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
310601
Florence Besse
Institute de Biologie Valrose (iBV) (http://ibv.unice.fr/)
me31B
C-terminal mTomato tag
DDX6
Dhh1p/Me31b
DmRH3
ME31B
Mat31B
Maternal expression at 31B
Me31B
Me31b
P-body marker
knock-in
l(2)k06607
mTomato
maternal expression at 31B
me31B
me31b
see publication
The mTomato sequence was inserted via Crispr/Cas9 in the me31B locus, at the C-terminus of the me31B coding sequence, using the strategy described in Kina et al., 2019 (DOI: 10.1111/dgd.12607). No selection marker was used. Phenotype: white eyes, larvae mTomato positive. This line can be used as a P-body marker, but not to assess granule material properties. It contains a 12 aa deletion at the end of Me31B coding sequence and the mTomato tag may affect granule material properties.
maternal expression at 31B
P-body marker
knock-in
mTomato
C-terminal mTomato tag
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310800
310800
AD-STOCK
Tagged Construct
y[1],w[1118]; P{CG10068-GFP}
CG10068 (orthologous to human Elmod) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
310800
Alexander Dammermann
Max Perutz Labs, University of Vienna
CG10068
Elmod-GFP
GFP
N-terminal
Ubq promoter
tag
CG10068 (orthologous to human Elmod) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
tag
Elmod-GFP
Ubq promoter
GFP
N-terminal
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310801
310801
AD-STOCK
Tagged Construct
y[1],w[1118]; P{CG10068-GFP}
CG10068 (orthologous to human Elmod) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
310801
Alexander Dammermann
Max Perutz Labs, University of Vienna
CG10068
C-terminal
Elmod-GFP
GFP
Ubq promoter
tag
CG10068 (orthologous to human Elmod) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
GFP
tag
C-terminal
Ubq promoter
Elmod-GFP
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310802
310802
AD-STOCK
Tagged Construct
y[1],w[1118]; P{nmdyn-D7-GFP}
CG8362 (orthologous to human NME7) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
310802
Alexander Dammermann
Max Perutz Labs, University of Vienna
nmdyn-D7
CG10068
GFP
N-terminal
NME7-GFP
Ubq promoter
nmdyn-D7
tag
CG8362 (orthologous to human NME7) tagged at N-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
nmdyn-D7
tag
Ubq promoter
N-terminal
NME7-GFP
GFP
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310803
310803
AD-STOCK
Tagged Construct
y[1],w[1118]; P{nmdyn-D7-GFP}
CG8362 (orthologous to human NME7) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
310803
Alexander Dammermann
Max Perutz Labs, University of Vienna
nmdyn-D7
C-terminal
CG10068
GFP
NME7-GFP
Ubq promoter
nmdyn-D7
tag
CG8362 (orthologous to human NME7) tagged at C-terminal with GFP under Ubq promoter. Construct inserted in genome via P-element integration.
nmdyn-D7
NME7-GFP
tag
C-terminal
Ubq promoter
GFP
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310804
310804
AD-STOCK
Tagged Construct
y[1],w[1118]; Rcd4-GFP
A GFP tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
310804
Alexander Dammermann
Max Perutz Labs, University of Vienna
Rcd4
BcDNA:SD16838
GFP
N-terminal
RCD4-GFP
Rcd4
Reduction in Cnn Dots 4
Reduction in Cnn dots 4
rcd4
tag
A GFP tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
Reduction in Cnn dots 4
N-terminal
RCD4-GFP
GFP
tag
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310805
310805
AD-STOCK
Tagged Construct
y[1],w[1118]; Rcd4-mCherry
An mCherry tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
310805
Alexander Dammermann
Max Perutz Labs, University of Vienna
Rcd4
BcDNA:SD16838
N-terminal
RCD4-mCherry
Rcd4
Reduction in Cnn Dots 4
Reduction in Cnn dots 4
mCherry
rcd4
tag
An mCherry tag has been inserted into the N-terminal end of the endogenous CG17295 (orthologous to human RCD4) coding sequence via CRISPR / gene targeting by homologous recombination.
Reduction in Cnn dots 4
mCherry
tag
N-terminal
RCD4-mCherry
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310806
310806
AD-STOCK
Deletion
y[1],w[1118]; Cep97-delta
Deletion Cep97 with ends-out method. Complete removal of CDS, clone II-6.
310806
Alexander Dammermann
Max Perutz Labs, University of Vienna
Cep97
CT13231
Centrosomal protein 97kDa
Cep97
DCep97
cep97
ends-out deletion
Deletion Cep97 with ends-out method. Complete removal of CDS, clone II-6.
Centrosomal protein 97kDa
ends-out deletion
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310807
310807
AD-STOCK
Tagged Construct
y[1],w[1118]; P{Cep97-GFP}
A genomic fragment (from the RP98-06H02 BAC clone) that encompasses the Cep97 transcription unit (includes 3.5kb upstream and 4.2kb downstream regulatory sequence). The coding sequence is tagged at the C-terminal end with GFP and construct inserted on chromsome 2 via P element insertion.
310807
Alexander Dammermann
Max Perutz Labs, University of Vienna
Cep97
C-terminal
CT13231
Centrosomal protein 97kDa
Cep97
Cep97-GFP
DCep97
GFP
cep97
tag
A genomic fragment (from the RP98-06H02 BAC clone) that encompasses the Cep97 transcription unit (includes 3.5kb upstream and 4.2kb downstream regulatory sequence). The coding sequence is tagged at the C-terminal end with GFP and construct inserted on chromsome 2 via P element insertion.
Centrosomal protein 97kDa
C-terminal
Cep97-GFP
GFP
tag
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310813
310813
AD-STOCK
Deletion
y[1],w[1118]; CG6191-delta
CG6191 (orthologous to human CABLES1 and CABLES2) deletion created via CRISPR using 2 guide RNAs. Deletions confirmed by PCR.
310813
Alexander Dammermann
Max Perutz Labs, University of Vienna
CG6191
BcDNA:GH06193
CABLES1
CABLES2
CRISPR
Dromel_CG6191_FBtr0087678_mORF
cables
deletion
CG6191 (orthologous to human CABLES1 and CABLES2) deletion created via CRISPR using 2 guide RNAs. Deletions confirmed by PCR.
CABLES1
CABLES2
deletion
CRISPR
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310817
310817
AD-STOCK
Tagged Construct
y[1],w[1118]; P{CG10137-GFP}
CG10137 (orthologous to human CEP104) tagged at C-terminal with GFP via pUBQ-vector and gateway cloning. Construct inserted in genome via P-element integration.
310817
Alexander Dammermann
Max Perutz Labs, University of Vienna
CG10137
C-terminal
CEP104
GFP
P-element insertion
pUBQ vector
tag
CG10137 (orthologous to human CEP104) tagged at C-terminal with GFP via pUBQ-vector and gateway cloning. Construct inserted in genome via P-element integration.
P-element insertion
tag
GFP
CEP104
pUBQ vector
C-terminal
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