J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 313115 |
313115
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JB-STOCK
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mutant alleles
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w; If/CyO; vreteno[delta1]/TM3,Ser;
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created by imprecise P-Element excision (using Bloomington stock #22204)
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313115
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vret
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empty
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created by imprecise P-Element excision (using Bloomington stock #22204)
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| 313302 |
313302
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JB-STOCK
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Tagged construct
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w; ; 3xFLAG/V5/Precission/GFP_Nxf3 [attP2]/TM3,Sb;
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Pacman CH322-97E07 containing the Nxf3 locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB399; Glycerol #670; tag probably interferes with function of Nxf3
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313302
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Nxf3
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empty
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Pacman CH322-97E07 containing the Nxf3 locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB399; Glycerol #670; tag probably interferes with function of Nxf3
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| 313531 |
313531
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JB-STOCK
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mutant alleles
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w[1118];; ago3 [g1]/TM3, Sb;
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ago3 Cas9 allele (guide RNA1; KS1-1m5-4); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: CATCTCGGTCGGCCG, ago3g1: CA−−−−−−−−−−CCG (-10)
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313531
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Ago3 KS1-1m5-4
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empty
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ago3 Cas9 allele (guide RNA1; KS1-1m5-4); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: CATCTCGGTCGGCCG, ago3g1: CA−−−−−−−−−−CCG (-10)
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| 313587 |
313587
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JB-STOCK
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RNAi lines
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w; pGLKD>aub_sh1 [attP33], nanos>GFP-Piwi_vasa3'UTR [attP40]/CyO; pV22>ago3_sh [attP2]/TM3, Ser;
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based on stocks KS6, TRiP GL00117, and aub_sh construct (plasmid JB144; pGLKD vector from Dominik Handler) inserted in attP33 (same plasmid used in stock 402);
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313587
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aub/ago3
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empty
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based on stocks KS6, TRiP GL00117, and aub_sh construct (plasmid JB144; pGLKD vector from Dominik Handler) inserted in attP33 (same plasmid used in stock 402);
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| 313598 |
313598
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JB-STOCK
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Crispr/Cas9
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w[1118]; iso2; Ly/TM3, Sb;
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w1118 iso1.1 with isogenized 2nd chrs and balanced on 3rd
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313598
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empty
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w1118 iso1.1 with isogenized 2nd chrs and balanced on 3rd
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| 313671 |
313671
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JB-STOCK
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GAL4 driver
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Cog-GAL4; internalGT-GAL4/CyO; nos-GAL4, vas-GFP/TM3,Ser;
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313671
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vasa
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empty
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| 313732 |
313732
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JB-STOCK
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mutant allele
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w; tub40 > alpha-catenin_cDNA[attP40]/CyO; alpha-catenin∆prom[m2-4]/TM3,Ser
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weak stock seq: TTGACTATCAGATACCTGTTACTCAGCTAGTGGCCAAG----GAAGAGCACTAGTAAAGATCTGGTCGTGTCCGTCTGTCCGTCCGTATGAAC
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313732
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Andersen et al., Nature 2017
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alpha-Catenin
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empty
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weak stock seq: TTGACTATCAGATACCTGTTACTCAGCTAGTGGCCAAG----GAAGAGCACTAGTAAAGATCTGGTCGTGTCCGTCTGTCCGTCCGTATGAAC
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| 313739 |
313739
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JB-STOCK
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mutant allele
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w; CG13741_A1-1/CyO;
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313739
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pan3a-m1-1; indel: 7; seq: CTTGCAAACCTACAtcccacatatcatccacatcGGGTGGCGATgaCGATGTTGAAGTTGAA
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CG13741
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empty
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| 313745 |
313745
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JB-STOCK
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mutant allele
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w; ; Nxf3_A2-2/TM3, Sb
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313745
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pan6a-m2-2; indel: -1; seq: GTTCACCGTG-ACCATCCGGTCACAGTGGCCACGCGGTGGTCCTCTTCGG
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nuclear RNA export factor 3
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empty
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| 313774 |
313774
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JB-STOCK
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Tagged construct
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w, moon_FS2∆28/FM7c; pNASA > LAP-TRF2s[attP40]/CyO; pNASA > Deadlock-vhhGFP[attP2]/TM3,Ser
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complete bypass stock
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313774
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Andersen et al., Nature 2017
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moonshiner
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empty
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complete bypass stock
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