J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 314045 |
314045
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JB-STOCK
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MTD;GFP reporter
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Cog-GAL4; NGT-GAL4/CyO; nos-GAL4, 3xFLAG_V5_Precission_GFP-Hel25E/TM3,Ser;
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314045
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ElMaghraby et al., 2019
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UAP56
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empty
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MTD;GFP reporter
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| 314047 |
314047
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JB-STOCK
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BoxB silencing reporter
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; pUASp>lambdaN-HA-Panx [attP40] tub>EGFP_5xBoxB_SV40 [attP33]/CyO;
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314047
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ElMaghraby et al., 2021
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BoxB silencing reporter
Panx |
empty
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BoxB silencing reporter
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| 313801 |
313801
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JB-STOCK
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tagged construct
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w; ; 3xFLAG_V5_Precission_GFP_smt3 [attP2]/TM3,Sb;
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Pacman CH322-158C23 containing the smt3 locus with N-terminal 3xFLAG_V5_Precission_GFP; Glycerol #915
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313801
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SUMO
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empty
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Pacman CH322-158C23 containing the smt3 locus with N-terminal 3xFLAG_V5_Precission_GFP; Glycerol #915
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| 313174 |
313174
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JB-STOCK
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RNAi lines
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w; ; pGLKD>BoYb_sh1(attP2)/TM3,Sb;
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAGCTGGAAGATGAAAGTAAAtagttatattcaagcataTTTACTTTCATCTTCCAGCTGgcg; used by Rippei
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313174
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BoYb
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empty
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in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAGCTGGAAGATGAAAGTAAAtagttatattcaagcataTTTACTTTCATCTTCCAGCTGgcg; used by Rippei
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| 313528 |
313528
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JB-STOCK
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mutant alleles
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w[1118]; piwi [g2.1]/CyO; iso3;
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piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)
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313528
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Piwi KS3-2m4-1
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empty
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piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)
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| 313530 |
313530
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JB-STOCK
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mutant alleles
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w[1118]; aub [g2]/CyO; iso3;
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aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)
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313530
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Aub KS2-2m1-1
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empty
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aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)
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| 313598 |
313598
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JB-STOCK
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Crispr/Cas9
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w[1118]; iso2; Ly/TM3, Sb;
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w1118 iso1.1 with isogenized 2nd chrs and balanced on 3rd
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313598
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empty
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w1118 iso1.1 with isogenized 2nd chrs and balanced on 3rd
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| 313780 |
313780
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JB-STOCK
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Tagged construct
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MTD; MTD/CyO; MTD, LAP-moon[attP2];
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313780
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moonshiner
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empty
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| 313968 |
313968
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JB-STOCK
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rescue construct
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w; ; nxf2[MA4-6], GFP-nxf2[delta 1st unit]/TM3,Sb;
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nxf2 mutant (Stock # 313682) containing Nxf2[delta 1st unit] coding sequence with the panoramix regulatory control regions (chr2R: 21,308,437-21,313,490) inserted into the attP154 landing site; published in Batki et al. 2019
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313968
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Batki, Schnabl et al, Nat Struct Mol Biol. 2019
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nuclear RNA export factor 2
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empty
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nxf2 mutant (Stock # 313682) containing Nxf2[delta 1st unit] coding sequence with the panoramix regulatory control regions (chr2R: 21,308,437-21,313,490) inserted into the attP154 landing site; published in Batki et al. 2019
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| 313984 |
313984
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JB-STOCK
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tagged endogenous genes
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; ; FLAG_V5_GFP_Nxf2 m11-1;
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Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;
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313984
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Batki, Schnabl et al, Nat Struct Mol Biol. 2019
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nuclear RNA export factor 2
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empty
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Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;
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| 313985 |
313985
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JB-STOCK
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tagged endogenous genes
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; ; FLAG_V5_GFP_Nxf2 m12-1;
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Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;
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313985
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Batki, Schnabl et al, Nat Struct Mol Biol. 2019
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nuclear RNA export factor 2
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empty
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Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;
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