J. Brennecke Stocks (JB-STOCK)
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    | Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 313703 | 
                                      313703  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      CRISPR line  
                                 | 
                                                            
                                      w; FLAG-nibbler[wt]/CyO; ;   
                                 | 
                                                            
                                      endogenous Nibbler allele with N-terminal 3xFLAG tag   
                                 | 
                                                            
                                      313703  
                                 | 
                                                            
                                      Hayashi & Schnabl, Nature, 2016  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      nibbler  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      endogenous Nibbler allele with N-terminal 3xFLAG tag   
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313705 | 
                                      313705  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      CRISPR line  
                                 | 
                                                            
                                      w; papi[1-5-2]; ;   
                                 | 
                                                            
                                      papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA  
                                 | 
                                                            
                                      313705  
                                 | 
                                                            
                                      Hayashi & Schnabl, Nature, 2016  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      papi  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313706 | 
                                      313706  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      CRISPR line  
                                 | 
                                                            
                                      w; papi[4-1-1]/CyO; ;   
                                 | 
                                                            
                                      papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG  
                                 | 
                                                            
                                      313706  
                                 | 
                                                            
                                      Hayashi & Schnabl, Nature, 2016  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      papi  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313713 | 
                                      313713  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      Pacman recombineering  
                                 | 
                                                            
                                      w; If/CyO; 3xFLAG_V5_Precission_GFP_Nbr[attP2]/TM3, Ser;   
                                 | 
                                                            
                                      Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807  
                                 | 
                                                            
                                      313713  
                                 | 
                                                            
                                      Hayashi & Schnabl, Nature, 2016  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      nibbler  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313714 | 
                                      313714  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      Pacman recombineering  
                                 | 
                                                            
                                      Cog-GAL4; NGT-GAL4; nos-GAL4, Zuc_GFP_Precission_V5_3xFLAG[attP2]/TM3, Ser;   
                                 | 
                                                            
                                     | 
                                                            
                                      313714  
                                 | 
                                                            
                                      Hayashi & Schnabl, Nature, 2016  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      zucchini  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
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| 313715 | 
                                      313715  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      Pacman recombineering  
                                 | 
                                                            
                                      w; Papi_GFP_Precission_V5_3xFLAG[attP40]/CyO; Sb/TM3, Ser;   
                                 | 
                                                            
                                      Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB569; Glycerol #735  
                                 | 
                                                            
                                      313715  
                                 | 
                                                            
                                      Hayashi & Schnabl, Nature, 2016  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      papi  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB569; Glycerol #735  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313717 | 
                                      313717  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      tagged construct  
                                 | 
                                                            
                                      w; ; Nxt1_GFP_Precission_V5_3xFLAG [attP2]/TM3,Sb;  
                                 | 
                                                            
                                      Pacman CH322-123E19 containing the Nxt1 locus with C-terminal GFP_Precission_V5_3xFLAG; Glycerol #836  
                                 | 
                                                            
                                      313717  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
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                                      p15, DmNXT2  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      Pacman CH322-123E19 containing the Nxt1 locus with C-terminal GFP_Precission_V5_3xFLAG; Glycerol #836  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313722 | 
                                      313722  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      reporter  
                                 | 
                                                            
                                      UAS Dcr-2(X),hs-hid(Y); NGT-Gal4-VP16; nos-GAL4, nos>GFP_lacZ_HeT-A_500/Ser;  
                                 | 
                                                            
                                      germline HeT-A sensor; based on stock 537;target sequence shorter than the published one (500 nt), 2 hours instead of o/n staining  
                                 | 
                                                            
                                      313722  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      HeT-A  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      germline HeT-A sensor; based on stock 537;target sequence shorter than the published one (500 nt), 2 hours instead of o/n staining  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313723 | 
                                      313723  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      reporter  
                                 | 
                                                            
                                      UAS Dcr-2(X),hs-hid(Y); NGT-Gal4-VP16; nos-GAL4, nos>GFP_lacZ_HeT-A_700/Ser;  
                                 | 
                                                            
                                      germline HeT-A sensor; based on stock 537; target sequence shorter than the published one (700 nt), 2 hours instead of o/n staining  
                                 | 
                                                            
                                      313723  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      HeT-A  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      germline HeT-A sensor; based on stock 537; target sequence shorter than the published one (700 nt), 2 hours instead of o/n staining  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313725 | 
                                      313725  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      mutant allele  
                                 | 
                                                            
                                      w; cluster38C1∆LEFTprom[1-6]/CyO;  
                                 | 
                                                            
                                      seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT  
                                 | 
                                                            
                                      313725  
                                 | 
                                                            
                                      Andersen et al., Nature 2017  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      Leftmost piRNA cluster at chromosomal band 38C  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313726 | 
                                      313726  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      mutant allele  
                                 | 
                                                            
                                      w, moon_FS2∆28/FM7c; cluster38C1∆LEFTprom[1-6]/CyO;  
                                 | 
                                                            
                                      seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT  
                                 | 
                                                            
                                      313726  
                                 | 
                                                            
                                      Andersen et al., Nature 2017  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      Leftmost piRNA cluster at chromosomal band 38C  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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| 313727 | 
                                      313727  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      mutant allele  
                                 | 
                                                            
                                      w; cluster38C1∆RIGHTprom[1-1]/CyO;  
                                 | 
                                                            
                                      seq; AAATATCTTCGAGAAACTCTGGATCGCAGACTCA---GCGAGGTTCGACCCCAACTATCAGCATTTTTTTTA  
                                 | 
                                                            
                                      313727  
                                 | 
                                                            
                                      Andersen et al., Nature 2017  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                     | 
                                                            
                                      Leftmost piRNA cluster at chromosomal band 38C  
                                 | 
                                                            
                                      empty  
                                 | 
                                                            
                                     | 
                                                            
                                      seq; AAATATCTTCGAGAAACTCTGGATCGCAGACTCA---GCGAGGTTCGACCCCAACTATCAGCATTTTTTTTA  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
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