J. Brennecke Stocks (JB-STOCK)

JB-STOCK
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Items 157-168 of 260

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Name VDRC Id Library Sub-Category Genotype Comment Construct Id Publication Provider Scientist Provider Organization Gene Symbol FlyBase gene number Synonyms Sequence RRID Comment Gene Name Keywords Price (net) Add to Cart
313703
313703
JB-STOCK
CRISPR line
w; FLAG-nibbler[wt]/CyO; ;
endogenous Nibbler allele with N-terminal 3xFLAG tag
313703
Hayashi & Schnabl, Nature, 2016
nibbler
empty
endogenous Nibbler allele with N-terminal 3xFLAG tag
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313705
313705
JB-STOCK
CRISPR line
w; papi[1-5-2]; ;
papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA
313705
Hayashi & Schnabl, Nature, 2016
papi
empty
papi null allele (frameshift induced by Cas9); sequence: GACATTAGCGGCGTTCCCGccgaca-TAAGGCGGCTCGAGCTCTGCTTATCAA
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313706
313706
JB-STOCK
CRISPR line
w; papi[4-1-1]/CyO; ;
papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG
313706
Hayashi & Schnabl, Nature, 2016
papi
empty
papi null allele (frameshift induced by Cas9); sequence: AGCGGACTCAGGTGGCCAACGGC----ATCCGGGATTAGAGGACAGACAGAGGAACAGAAGCCCCAGAAGG
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313713
313713
JB-STOCK
Pacman recombineering
w; If/CyO; 3xFLAG_V5_Precission_GFP_Nbr[attP2]/TM3, Ser;
Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807
313713
Hayashi & Schnabl, Nature, 2016
nibbler
empty
Pacman CH322-18I04 containing the Nbr locus with N-terminal 3xFLAG_V5_Precission_GFP; plasmid JB677; Glycerol #807
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313714
313714
JB-STOCK
Pacman recombineering
Cog-GAL4; NGT-GAL4; nos-GAL4, Zuc_GFP_Precission_V5_3xFLAG[attP2]/TM3, Ser;
313714
Hayashi & Schnabl, Nature, 2016
zucchini
empty
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313715
313715
JB-STOCK
Pacman recombineering
w; Papi_GFP_Precission_V5_3xFLAG[attP40]/CyO; Sb/TM3, Ser;
Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB569; Glycerol #735
313715
Hayashi & Schnabl, Nature, 2016
papi
empty
Pacman CH322-41G09 containing the papi locus with C-terminal GFP_Precission_V5_3xFLAG; plasmid JB569; Glycerol #735
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313717
313717
JB-STOCK
tagged construct
w; ; Nxt1_GFP_Precission_V5_3xFLAG [attP2]/TM3,Sb;
Pacman CH322-123E19 containing the Nxt1 locus with C-terminal GFP_Precission_V5_3xFLAG; Glycerol #836
313717
p15, DmNXT2
empty
Pacman CH322-123E19 containing the Nxt1 locus with C-terminal GFP_Precission_V5_3xFLAG; Glycerol #836
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313722
313722
JB-STOCK
reporter
UAS Dcr-2(X),hs-hid(Y); NGT-Gal4-VP16; nos-GAL4, nos>GFP_lacZ_HeT-A_500/Ser;
germline HeT-A sensor; based on stock 537;target sequence shorter than the published one (500 nt), 2 hours instead of o/n staining
313722
HeT-A
empty
germline HeT-A sensor; based on stock 537;target sequence shorter than the published one (500 nt), 2 hours instead of o/n staining
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313723
313723
JB-STOCK
reporter
UAS Dcr-2(X),hs-hid(Y); NGT-Gal4-VP16; nos-GAL4, nos>GFP_lacZ_HeT-A_700/Ser;
germline HeT-A sensor; based on stock 537; target sequence shorter than the published one (700 nt), 2 hours instead of o/n staining
313723
HeT-A
empty
germline HeT-A sensor; based on stock 537; target sequence shorter than the published one (700 nt), 2 hours instead of o/n staining
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313725
313725
JB-STOCK
mutant allele
w; cluster38C1∆LEFTprom[1-6]/CyO;
seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
313725
Andersen et al., Nature 2017
Leftmost piRNA cluster at chromosomal band 38C
empty
seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
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313726
313726
JB-STOCK
mutant allele
w, moon_FS2∆28/FM7c; cluster38C1∆LEFTprom[1-6]/CyO;
seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
313726
Andersen et al., Nature 2017
Leftmost piRNA cluster at chromosomal band 38C
empty
seq: GACTCCTGCAAGCAATGAGCGATACCCCA---AGAAGAGCACTAGTAAATGAAGTGGAGACCAGGGT
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313727
313727
JB-STOCK
mutant allele
w; cluster38C1∆RIGHTprom[1-1]/CyO;
seq; AAATATCTTCGAGAAACTCTGGATCGCAGACTCA---GCGAGGTTCGACCCCAACTATCAGCATTTTTTTTA
313727
Andersen et al., Nature 2017
Leftmost piRNA cluster at chromosomal band 38C
empty
seq; AAATATCTTCGAGAAACTCTGGATCGCAGACTCA---GCGAGGTTCGACCCCAACTATCAGCATTTTTTTTA
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