J. Brennecke Stocks (JB-STOCK)
JB-STOCK
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
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| 313524 |
313524
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JB-STOCK
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RNAi lines
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w; ; pW20>Su(var)3-9_sh2 [attP2]/TM3, Sb;
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most likely in pW20 vector, but possibly also in pGLKD vector from Dominik Handler; oligo sequence: CTAGCAGTCAGCTCTACGAGATATACATATAGTTATATTCAAGCATATATGTATATCTCGTAGAGCTGGCG
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313524
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Su(var)3-9
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empty
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most likely in pW20 vector, but possibly also in pGLKD vector from Dominik Handler; oligo sequence: CTAGCAGTCAGCTCTACGAGATATACATATAGTTATATTCAAGCATATATGTATATCTCGTAGAGCTGGCG
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| 313527 |
313527
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JB-STOCK
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mutant alleles
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w[1118]; piwi [g1]/CyO; iso3;
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piwi Cas9 allele (guide RNA1; KS3-1m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; it carries the following frameshift sequence: w1118: ATCCTCGTATAGAGGC piwi_g1:ATCCTCG−−TAGAGGC (-2)
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313527
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Piwi KS3-1m1-1
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empty
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piwi Cas9 allele (guide RNA1; KS3-1m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; it carries the following frameshift sequence: w1118: ATCCTCGTATAGAGGC piwi_g1:ATCCTCG−−TAGAGGC (-2)
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| 313528 |
313528
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JB-STOCK
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mutant alleles
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w[1118]; piwi [g2.1]/CyO; iso3;
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piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)
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313528
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Piwi KS3-2m4-1
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empty
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piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)
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| 313529 |
313529
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JB-STOCK
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mutant alleles
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w[1118]; aub [g1]/CyO; iso3;
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aub Cas9 allele (guide RNA1; KS2-1m3-2); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: ACGACTGCGACGGTC, aub_g1:ACGAC−−−−ACGGTC (-4)
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313529
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Aub KS2-1m3-2
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empty
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aub Cas9 allele (guide RNA1; KS2-1m3-2); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: ACGACTGCGACGGTC, aub_g1:ACGAC−−−−ACGGTC (-4)
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| 313530 |
313530
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JB-STOCK
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mutant alleles
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w[1118]; aub [g2]/CyO; iso3;
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aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)
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313530
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Aub KS2-2m1-1
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empty
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aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)
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| 313532 |
313532
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JB-STOCK
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mutant alleles
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w[1118];; ago3 [g2]/TM3, Sb;
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ago3 Cas9 allele (guide RNA2; KS1-2m7-3); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: CGAGGACGTGCTAGG, ago3_g2: CG−−−−−−−−−−AGG (-10)
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313532
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Ago3 KS1-2m7-3
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empty
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ago3 Cas9 allele (guide RNA2; KS1-2m7-3); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: CGAGGACGTGCTAGG, ago3_g2: CG−−−−−−−−−−AGG (-10)
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| 313533 |
313533
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JB-STOCK
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mutant alleles
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w[1118];; ago3 [g3]/TM3, Sb;
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ago3 Cas9 allele (guide RNA3; KS1-3m12-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: TAGAAGTTTGGCCTG, ago3_g3: TAGAA−−−−−GCCTG (-5)
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313533
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Ago3 KS1-3m12-1
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empty
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ago3 Cas9 allele (guide RNA3; KS1-3m12-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: TAGAAGTTTGGCCTG, ago3_g3: TAGAA−−−−−GCCTG (-5)
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| 313535 |
313535
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JB-STOCK
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mutant alleles
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w[1118];; ago3 [g4]/TM3, Sb;
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ago3 Cas9 allele (guide RNA4; KS1-4m2-2); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: CGAATACAAAGGTGG, ago3_g4: CGAATAC-AAGGTGG (-1)
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313535
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Ago3 KS1-4m2-2
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empty
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ago3 Cas9 allele (guide RNA4; KS1-4m2-2); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); 2nd chromosome is not isogenic; stock becomes over time homozygous partially fertile; it carries the following frameshift sequence: w1118: CGAATACAAAGGTGG, ago3_g4: CGAATAC-AAGGTGG (-1)
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| 313538 |
313538
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JB-STOCK
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piRNA biogenesis reporter
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w; piRNA-biogenesis-reporter #6 [attp40]/CyO; ;
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Gblock cloned into plasmid JB634 (trigger sensor plasmid version 2 from Dominik Handler); Gblock: trigger 6 (trigger piRNA: robust and Ago3 biased);
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313538
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empty
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Gblock cloned into plasmid JB634 (trigger sensor plasmid version 2 from Dominik Handler); Gblock: trigger 6 (trigger piRNA: robust and Ago3 biased);
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| 313583 |
313583
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JB-STOCK
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RNAi lines
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w; pGLKD>aub_sh1 [attP40]; pV22>ago3_sh [attP2];
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based on TRiP GL00117 and aub_sh construct (plasmid JB144; pGLKD vector from Dominik Handler) inserted in attP40 (same plasmid used in stock 402);
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313583
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aub/ago3
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empty
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based on TRiP GL00117 and aub_sh construct (plasmid JB144; pGLKD vector from Dominik Handler) inserted in attP40 (same plasmid used in stock 402);
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| 313584 |
313584
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JB-STOCK
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marker
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w; nanos>GFP-Piwi_vasa3'UTR [attP40]/CyO; Sb/TM3, Ser;
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construct carries a nanos promoter driving the Piwi CDS with a vasa 3'UTR; expression restricted to germline cells;
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313584
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piwi
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empty
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construct carries a nanos promoter driving the Piwi CDS with a vasa 3'UTR; expression restricted to germline cells;
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| 313585 |
313585
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JB-STOCK
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RNAi lines
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w; nanos>GFP-Piwi_vasa3'UTR [attP40]/CyO; pGLKD>aub_sh1 [attP2]/TM3, Ser;
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based on stocks 402 and KS6
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313585
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aub
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empty
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based on stocks 402 and KS6
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