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                    - Category J. Brennecke Stocks (JB-STOCK) Remove This Item
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | FlyBase gene number | Synonyms | Sequence | RRID | Comment | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 313174 | 
                                      313174  
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                                      JB-STOCK  
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                                      RNAi lines  
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                                      w; ; pGLKD>BoYb_sh1(attP2)/TM3,Sb;   
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                                      in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAGCTGGAAGATGAAAGTAAAtagttatattcaagcataTTTACTTTCATCTTCCAGCTGgcg; used by Rippei  
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                                      313174  
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                                      BoYb  
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                                      empty  
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                                      in Dominik Handler's germline knockdown vector (plasmid JB227); sh-oligo sequence: ctagcagtCAGCTGGAAGATGAAAGTAAAtagttatattcaagcataTTTACTTTCATCTTCCAGCTGgcg; used by Rippei  
                                 | 
                                                            
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                                                        Sign In to show prices | Temporarily unavailable | |||
| 313528 | 
                                      313528  
                                 | 
                                                            
                                      JB-STOCK  
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                                      mutant alleles  
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                                      w[1118]; piwi [g2.1]/CyO; iso3;  
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                                      piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)  
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                                      313528  
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                                      Piwi KS3-2m4-1  
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                                      empty  
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                                      piwi Cas9 allele (guide RNA2; KS3-2m4-1) generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); stock is difficult to keep; eliminate all non-CyO flies each generation; this stock replaces the published strain piwi [g2], which died; it carries the following frameshift sequence: w1118: GAAGCCTCTATACGAGG piwi_g2.1:GAAGCCTCT−−CGAGG (-2)  
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                                     | 
                                                        Sign In to show prices | Temporarily unavailable | |||
| 313530 | 
                                      313530  
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                                      JB-STOCK  
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                                      mutant alleles  
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                                      w[1118]; aub [g2]/CyO; iso3;  
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                                      aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)  
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                                      313530  
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                                      Aub KS2-2m1-1  
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                                      empty  
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                                      aub Cas9 allele (guide RNA2; KS2-2m1-1); generated in the w[1118] stock with isogenized 2nd and 3rd chromosomes (stock KS18); it carries the following frameshift sequence: w1118: AGGGATCCTGGGCGGC, aub_g2:AGGG−−−−−−−−CGGC (-8)  
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                                                        Sign In to show prices | Temporarily unavailable | |||
| 313598 | 
                                      313598  
                                 | 
                                                            
                                      JB-STOCK  
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                                      Crispr/Cas9  
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                                      w[1118]; iso2; Ly/TM3, Sb;  
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                                      w1118 iso1.1 with isogenized 2nd chrs and balanced on 3rd  
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                                      313598  
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                                      empty  
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                                      w1118 iso1.1 with isogenized 2nd chrs and balanced on 3rd  
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                                                        Sign In to show prices | Temporarily unavailable | |||
| 313732 | 
                                      313732  
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                                      JB-STOCK  
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                                      mutant allele  
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                                      w; tub40 > alpha-catenin_cDNA[attP40]/CyO; alpha-catenin∆prom[m2-4]/TM3,Ser  
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                                      weak stock seq: TTGACTATCAGATACCTGTTACTCAGCTAGTGGCCAAG----GAAGAGCACTAGTAAAGATCTGGTCGTGTCCGTCTGTCCGTCCGTATGAAC  
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                                      313732  
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                                      Andersen et al., Nature 2017  
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                                      alpha-Catenin  
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                                      empty  
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                                      weak stock seq: TTGACTATCAGATACCTGTTACTCAGCTAGTGGCCAAG----GAAGAGCACTAGTAAAGATCTGGTCGTGTCCGTCTGTCCGTCCGTATGAAC  
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                                                        Sign In to show prices | Temporarily unavailable | ||
| 313780 | 
                                      313780  
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                                      JB-STOCK  
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                                      Tagged construct  
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                                      MTD; MTD/CyO; MTD, LAP-moon[attP2];  
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                                      313780  
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                                      moonshiner  
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                                      empty  
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                                                        Sign In to show prices | Temporarily unavailable | ||
| 313968 | 
                                      313968  
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                                      JB-STOCK  
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                                      rescue construct  
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                                      w; ; nxf2[MA4-6], GFP-nxf2[delta 1st unit]/TM3,Sb;  
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                                      nxf2 mutant (Stock # 313682) containing Nxf2[delta 1st unit] coding sequence with the panoramix regulatory control regions (chr2R: 21,308,437-21,313,490) inserted into the attP154 landing site; published in Batki et al. 2019  
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                                      313968  
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                                      Batki, Schnabl et al, Nat Struct Mol Biol. 2019  
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                                      nuclear RNA export factor 2  
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                                      empty  
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                                      nxf2 mutant (Stock # 313682) containing Nxf2[delta 1st unit] coding sequence with the panoramix regulatory control regions (chr2R: 21,308,437-21,313,490) inserted into the attP154 landing site; published in Batki et al. 2019  
                                 | 
                                                            
                                     | 
                                                            
                                     | 
                                                        Sign In to show prices | Temporarily unavailable | ||
| 313984 | 
                                      313984  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      tagged endogenous genes  
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                                      ; ; FLAG_V5_GFP_Nxf2 m11-1;  
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                                      Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;   
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                                      313984  
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                                      Batki, Schnabl et al, Nat Struct Mol Biol. 2019  
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                                      nuclear RNA export factor 2  
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                                      empty  
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                                      Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;   
                                 | 
                                                            
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                                     | 
                                                        Sign In to show prices | Temporarily unavailable | ||
| 313985 | 
                                      313985  
                                 | 
                                                            
                                      JB-STOCK  
                                 | 
                                                            
                                      tagged endogenous genes  
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                                      ; ; FLAG_V5_GFP_Nxf2 m12-1;  
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                                      Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;   
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                                      313985  
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                                      Batki, Schnabl et al, Nat Struct Mol Biol. 2019  
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                                      nuclear RNA export factor 2  
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                                      empty  
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                                      Guide: CAAAGTCCAGCACTCTCATC, Donor: N-terminal 3xFLAG_V5_Precission_loxP_GFP tag;   
                                 | 
                                                            
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                                     | 
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