Heidelberg CFD CRISPR Library

For more information about our Heidelberg CFD CRISPR Library, please check out our information page.

For explanations of fields, please checkout out our field definitions.

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7 Items

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Name VDRC Id Library Sub-Category Genotype Comment Construct Id Publication Provider Scientist Provider Organization Gene Symbol FlyBase gene number Synonyms HDtools ID Target sgRNA1 Target sgRNA2 Insertion Site Vector Sequence RRID Comment Name Gene Name Keywords Price (net) Add to Cart
340007
340007
HDtools-Cas9
Cas9
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; ;PBac{y[+]-attP-3B=UAS-uMCas9}VK00033
for medium Cas9 levels
340007
UAS-uMCas9
HD_CFDtools008
attPVK00033
pUASgattB
for medium Cas9 levels
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; ;PBac{y[+]-attP-3B=UAS-uMCas9}VK00033
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340020
340020
HDtools-Cas9
Gal4 Cas9
w[*]; PBac{y[+]-attP-3B=UAS-uMCas9}VK00033 P{Gal4}hh-Gal4/TM6B
hhGal4 UAS-uMCas9 recombined on 3rd
340020
hh-Gal4 UAS-uMCas9(VK33)
HD_CFDtools021
attPVK00033
pUASgattB
hhGal4 UAS-uMCas9 recombined on 3rd
w[*]; PBac{y[+]-attP-3B=UAS-uMCas9}VK00033 P{Gal4}hh-Gal4/TM6B
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340021
340021
HDtools-Cas9
Cas9
w[*];; M{3xP3-RFP.attP=UAS-uMCas9}ZH-86Fb
340021
UAS-uMCas9(ZH-86Fb)
HD_CFDtools022
ZH-86Fb
pUASgattB
w[*];; M{3xP3-RFP.attP=UAS-uMCas9}ZH-86Fb
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340104
340104
HDtools-12a
CRISPR nuclease
w[*]; P{GAL4::VP16-nos.UTR}CG6325MVD1, PBac{y[+]-attP-3B=UAS-uMCas12a+}VK00033/TM6B
nos-Gal4VP16 UAS-uMCas12a+. Germline specific expression of Cas12a+.
340104
Fillip Port and Michael Boutros
DKFZ, Heidelberg
CRISPR
Cas12a
knockout
nuclease
340104
nos-Gal4VP16 UAS-uMCas12a+. Germline specific expression of Cas12a+.
knockout
nuclease
Cas12a
CRISPR
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340109
340109
HDtools-12a
CRISPR nuclease
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; ;PBac{y[+]-attP-3B=UAS-uMCas12a+}VK00033
UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
340109
Fillip Port and Michael Boutros
DKFZ, Heidelberg
CRISPR
Cas12a
knockout
nuclease
340109
UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
knockout
CRISPR
nuclease
Cas12a
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349800
349800
HD-CFD-Base-Editing
Base Editor
P{ry[+t7.2]=hsFLP}12, y[1] w[*]; ;PBac{y[+]-attP-3B=UAST-DBEevoCDA1}VK00033
UAST-DBEevoCDA1. GAL4 dependent expression of DBEevoCDA1.
349800
Fillip Port and Michael Boutros
DKFZ, Heidelberg
CRISPR
base editing
mutations
UAST-DBEevoCDA1. GAL4 dependent expression of DBEevoCDA1.
base editing
CRISPR
mutations
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349805
349805
HD-CFD-Base-Editing
Base Editor
w[*]; ;PBac{y[+]-attP-3B=UAST-FRT-GFP-FRT-uMDBEevoCDA1}VK00033
UAST-FRT-GFP-FRT-uMDBEevoCDA1. GAl4 and FLP dependent expression of DBEevoCDA1. Expressing cells lose GFP expression.
349805
Fillip Port and Michael Boutros
DKFZ, Heidelberg
CRISPR
base editing
mutations
UAST-FRT-GFP-FRT-uMDBEevoCDA1. GAl4 and FLP dependent expression of DBEevoCDA1. Expressing cells lose GFP expression.
CRISPR
mutations
base editing
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