Heidelberg CFD CRISPR Library
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | Synonyms | HD_CFD ID | HDtools ID | Target sgRNA1 | Target sgRNA2 | Insertion Site | Vector | RRID | Comment | Name | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 343843 |
343843
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0844}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0051205.
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343843
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CG31205
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CRISPR
Cas12a HD12aCFD0844 knockout sgRNA |
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343843
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0051205.
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knockout
CRISPR sgRNA Cas12a |
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| 343844 |
343844
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0845}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0003205.
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343844
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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Ras85D
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C-ras1
CRISPR Cas12a D-Ras D-Ras1 D-ras-1 D-ras1 DRAS1 DRas DRas85D/Ras Dm Ras1 Dmras85D Dras Dras1 Dras85D E(faf) E(sev)3C EK3-4 Enhancer of faf HD12aCFD0845 P08646 RAS RAS1 RAS85D R |
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343844
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0003205.
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Ras oncogene at 85D
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Cas12a
CRISPR knockout sgRNA |
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| 340023 |
340023
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HD-CFD-Cas9
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CRISPR nuclease
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w[*]; P{y[+t7.7] w[+mC]=GRACE}attP40/CyO; PBac{y[+]-attP-3B=UAST-sgRNA-Cas9_GRACE}VK00033
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GRACE_Cas9. Tracing of Cas9 activity through GFP expression.
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340023
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340023
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GRACE_Cas9. Tracing of Cas9 activity through GFP expression.
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CRISPR
Cas12a knockout nuclease |
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| 340100 |
340100
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HDtools-12a
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CRISPR nuclease
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=act5C-Cas12a+}attP40/CyO, P{GFP, w[-]}
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Act-Cas12a+. Expression of Cas12a+ under act5C promoter (near ubiquitous).
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340100
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340100
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Act-Cas12a+. Expression of Cas12a+ under act5C promoter (near ubiquitous).
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Cas12a
knockout nuclease CRISPR |
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| 340101 |
340101
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HDtools-12a
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CRISPR nuclease
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAST-uMCas12a+}attP40/CyO, P{GFP, w[-]}
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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340101
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340101
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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CRISPR
knockout Cas12a nuclease |
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| 340102 |
340102
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HDtools-12a
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CRISPR nuclease
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w[*]; P{y[+t7.7] w[+mC]=UAST-FRT-GFP-FRT-uMCas12a+}attP40/CyO, P{GFP, w[-]}
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UAST-FRT-GFP-FRT-uMCas12a+. GAL4 and FLP dependent expression of Cas12a+. Expressing cells lose GFP expression.
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340102
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340102
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UAST-FRT-GFP-FRT-uMCas12a+. GAL4 and FLP dependent expression of Cas12a+. Expressing cells lose GFP expression.
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nuclease
CRISPR knockout Cas12a |
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| 340103 |
340103
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HDtools-12a
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CRISPR nuclease
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w[*]; P{y[+t7.7] w[+mC]=GRACE}attP40/CyO; PBac{y[+]-attP-3B=U6:3-sgRNA-Cas12a_GRACE}VK00033
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GRACE_Cas12a. Tracing of Cas12a activity through GFP expression.
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340103
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340103
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GRACE_Cas12a. Tracing of Cas12a activity through GFP expression.
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Cas12a
CRISPR nuclease knockout |
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| 340104 |
340104
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HDtools-12a
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CRISPR nuclease
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w[*]; P{GAL4::VP16-nos.UTR}CG6325MVD1, PBac{y[+]-attP-3B=UAS-uMCas12a+}VK00033/TM6B
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nos-Gal4VP16 UAS-uMCas12a+. Germline specific expression of Cas12a+.
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340104
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340104
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nos-Gal4VP16 UAS-uMCas12a+. Germline specific expression of Cas12a+.
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knockout
nuclease Cas12a CRISPR |
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| 340105 |
340105
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HDtools-12a
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CRISPR nuclease
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w[*]; P{y[+t7.7] w[+mC]=UAS-uMCas12a+}attP40; P{Gal4}hh-Gal4/TM6B
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hh-Gal4 UAST-uMCas12a+. hh-Gal4 driving expression of Cas12a+.
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340105
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340105
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hh-Gal4 UAST-uMCas12a+. hh-Gal4 driving expression of Cas12a+.
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Cas12a
nuclease knockout CRISPR |
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| 340106 |
340106
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HDtools-12a
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CRISPR nuclease
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w[*]; P{y[+t7.7] w[+mC]=UAS-uMCas12a+}attP40; P{Gal4}pdm2-Gal4/TM6B
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pdm2-Gal4 UAST-uMcas12a+. pdm2-Gal4 driving expression of Cas12a+.
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340106
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340106
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pdm2-Gal4 UAST-uMcas12a+. pdm2-Gal4 driving expression of Cas12a+.
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knockout
Cas12a nuclease CRISPR |
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| 340107 |
340107
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HDtools-12a
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CRISPR nuclease
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7]=UAST-uMCas12a+}JK22C/CyO, P{GFP, w[-]}
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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340107
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340107
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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nuclease
CRISPR knockout Cas12a |
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| 340108 |
340108
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HDtools-12a
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CRISPR nuclease
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y[1] M{w[+mC]=UAST-uMCas12a+.P}ZH-2A w[*]; wg[Sp-1]/CyO
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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340108
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340108
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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knockout
nuclease Cas12a CRISPR |
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