Heidelberg CFD CRISPR Library
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| Name | VDRC Id | Library | Sub-Category | Genotype | Comment | Construct Id | Publication | Provider Scientist | Provider Organization | Gene Symbol | Synonyms | HD_CFD ID | HDtools ID | Target sgRNA1 | Target sgRNA2 | Insertion Site | Vector | Sequence | RRID | Comment | Name | Gene Name | Keywords | Price (net) | Add to Cart | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 343840 |
343840
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0841}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0032240.
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343840
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CG17768
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BcDNA:RE35747
CG31990 CG5356 CRISPR Cas12a HD12aCFD0841 knockout sgRNA |
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343840
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0032240.
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Cas12a
CRISPR knockout sgRNA |
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| 343841 |
343841
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0842}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0031244.
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343841
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CG11601
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CRISPR
Cas12a HD12aCFD0842 knockout sgRNA |
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343841
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0031244.
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CRISPR
knockout sgRNA Cas12a |
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| 343842 |
343842
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0843}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0053679.
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343842
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CG33679
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BP1003
CRISPR Cas12a HD12aCFD0843 knockout sgRNA |
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343842
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0053679.
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Cas12a
sgRNA CRISPR knockout |
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| 343843 |
343843
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0844}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0051205.
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343843
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CG31205
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CRISPR
Cas12a HD12aCFD0844 knockout sgRNA |
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343843
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0051205.
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knockout
CRISPR sgRNA Cas12a |
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| 343844 |
343844
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HD-CFD-12a
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sgRNA
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=HD12aCFD0845}attP40/CyO, P{GFP, w[-]}
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0003205.
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343844
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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Ras85D
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C-ras1
CRISPR Cas12a D-Ras D-Ras1 D-ras-1 D-ras1 DRAS1 DRas DRas85D/Ras Dm Ras1 Dmras85D Dras Dras1 Dras85D E(faf) E(sev)3C EK3-4 Enhancer of faf HD12aCFD0845 P08646 RAS RAS1 RAS85D R |
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343844
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Expression of multiplexed sgRNAs from a U6:3 promoter targeting gene FBgn0003205.
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Ras oncogene at 85D
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Cas12a
CRISPR knockout sgRNA |
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| 340100 |
340100
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HDtools-12a
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CRISPR nuclease
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=act5C-Cas12a+}attP40/CyO, P{GFP, w[-]}
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Act-Cas12a+. Expression of Cas12a+ under act5C promoter (near ubiquitous).
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340100
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340100
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Act-Cas12a+. Expression of Cas12a+ under act5C promoter (near ubiquitous).
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Cas12a
knockout nuclease CRISPR |
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| 340101 |
340101
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HDtools-12a
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CRISPR nuclease
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAST-uMCas12a+}attP40/CyO, P{GFP, w[-]}
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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340101
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340101
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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CRISPR
knockout Cas12a nuclease |
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| 340102 |
340102
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HDtools-12a
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CRISPR nuclease
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w[*]; P{y[+t7.7] w[+mC]=UAST-FRT-GFP-FRT-uMCas12a+}attP40/CyO, P{GFP, w[-]}
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UAST-FRT-GFP-FRT-uMCas12a+. GAL4 and FLP dependent expression of Cas12a+. Expressing cells lose GFP expression.
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340102
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340102
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UAST-FRT-GFP-FRT-uMCas12a+. GAL4 and FLP dependent expression of Cas12a+. Expressing cells lose GFP expression.
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nuclease
CRISPR knockout Cas12a |
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| 340107 |
340107
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HDtools-12a
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CRISPR nuclease
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7]=UAST-uMCas12a+}JK22C/CyO, P{GFP, w[-]}
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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340107
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
Cas12a knockout nuclease |
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340107
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UAST-uMCas12a+. GAL4 dependent expression of Cas12a+.
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nuclease
CRISPR knockout Cas12a |
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| 349801 |
349801
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HD-CFD-Base-Editing
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Base Editor
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=act5C-DBEevoAPOBEC1}attP40/CyO, P{GFP, w[-]}
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Act-DBEevoAPOBEC1. Expression of DBEevoAPOBEC1 under act5C promoter (near ubiquitous).
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349801
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
base editing mutations |
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Act-DBEevoAPOBEC1. Expression of DBEevoAPOBEC1 under act5C promoter (near ubiquitous).
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CRISPR
mutations base editing |
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| 349802 |
349802
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HD-CFD-Base-Editing
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Base Editor
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=UAST-DBEevoAPOBEC1}attP40/CyO, P{GFP, w[-]}
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UAST-DBEevoAPOBEC1. GAL4 dependent expression of DBEevoAPOBEC1.
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349802
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
base editing mutations |
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UAST-DBEevoAPOBEC1. GAL4 dependent expression of DBEevoAPOBEC1.
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base editing
mutations CRISPR |
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| 349803 |
349803
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HD-CFD-Base-Editing
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Base Editor
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P{ry[+t7.2]=hsFLP}12, y[1] w[*]; P{y[+t7.7] w[+mC]=act5C-DBEevoCDA1}attP40/CyO, P{GFP, w[-]}
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Act-DBEevoCDA1. Expression of DBEevoCDA1 under act5C promoter (near ubiquitous)
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349803
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Fillip Port and Michael Boutros
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DKFZ, Heidelberg
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CRISPR
base editing mutations |
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Act-DBEevoCDA1. Expression of DBEevoCDA1 under act5C promoter (near ubiquitous)
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CRISPR
mutations base editing |
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