DrosDel Immunity Panel

Name	VDRC Id	Library	Sub-Category	Genotype	Comment	Construct Id	Publication	Provider Scientist	Provider Organization	Gene Symbol	Synonyms	Comment	Gene Name	Keywords	Price (net)
349900	349900	DD-Im	mutants	w[1118]; ; Rel[E20]	BL1. A deletion of Relish (which also affects a nearby gene). The ebony marker of the original stock (Dan Hultmark‘s lab) was removed by recombination with an Oregon stock. The Rel[E20] mutation was then introgressed in the w[1118] DrosDel genetic background. Rel[E20] lack a functional Imd pathway and are susceptible to Gram negative bacterial infection. The transcription factor Relish may also be involved in the immune defence against viruses downstream of cGas-like, Sting and IKK (Cai et al., Curr Opin Immumol (2022)).	349900	Ferreira et al., PLoS Pathog (2014)	Bruno Lemaitre with the help of Mark Hanson	EPFL	Rel	DrosDel Immunity panel NF-KB NF-kappaB NF-κB NFkappaB NFκB Nuclear Factor-kappa-B p110 Nuclear factor NF-kappa-B p110 subunit REL RELI RELISH Rel Rel-p110 Rel/NF-kappaB Rel/NF-κB Rel1 Rel49 RelA Relish	BL1. A deletion of Relish (which also affects a nearby gene). The ebony marker of the original stock (Dan Hultmark‘s lab) was removed by recombination with an Oregon stock. The Rel[E20] mutation was then introgressed in the w[1118] DrosDel genetic background. Rel[E20] lack a functional Imd pathway and are susceptible to Gram negative bacterial infection. The transcription factor Relish may also be involved in the immune defence against viruses downstream of cGas-like, Sting and IKK (Cai et al., Curr Opin Immumol (2022)).	Relish	DrosDel Immunity panel	Sign In to show prices
349901	349901	DD-Im	mutants	w[1118]; ; spz[rm7]/TM6C, Sb[1]	BL2. spz[rm7] or spz[4] is a genetically null mutation in spz (generated by EMS during Nusslein-Volhard and Wieschaus screen). Several markers of the original stock (M317 Tubingen stock center) including ebony were removed by recombination (Lemaitre et al., Cell 1996). spz[rm7] are homozygous viable, female sterile. This mutation blocks the activation of the Toll pathway. Note that the Toll pathway might be activated independently of spz by spz[5] (Nonaka et al., Biochem Biophys Res Comm 2018)).	349901	Morisato & Anderson, Cell (1994)	Bruno Lemaitre with the help of Mark Hanson	EPFL	spz	CT19282 DrosDel Immunity panel Protein spaetzle precursor SPZ Spaetzle Spatzle Spatzle-1 Spz Spz-1 Spz1 mel(3)7 spaetzle spatzle spazle spaztle spz	BL2. spz[rm7] or spz[4] is a genetically null mutation in spz (generated by EMS during Nusslein-Volhard and Wieschaus screen). Several markers of the original stock (M317 Tubingen stock center) including ebony were removed by recombination (Lemaitre et al., Cell 1996). spz[rm7] are homozygous viable, female sterile. This mutation blocks the activation of the Toll pathway. Note that the Toll pathway might be activated independently of spz by spz[5] (Nonaka et al., Biochem Biophys Res Comm 2018)).	spatzle	DrosDel Immunity panel	Sign In to show prices
349907	349907	DD-Im	mutants	w[1118]; ; Sp7[SK6]	BL8. Null mutation affecting the Sp7/MP2 serine protease that regulates melanization in Drosophila, isogenized in the w[1118] DrosDel genetic background. Sp7 strongly blocks the blackening reaction at the injury site of larvae but has less effect in adults (Dudzic et al., 2015).	349907	Dudzic et al., BMC Biology (2015)	Bruno Lemaitre with the help of Mark Hanson	EPFL	Sp7	DrosDel Immunity panel MP1 MP2 MP2/sp7/PAE1 Melanization Protease 2 PAE PAE1 SP13 SP7 Serine protease 7 Serine protease-7 Sp 7 Sp7 anon-Ryu cSP7 proPO-AE prophenoloxidase-activating enzyme prophenoloxidas	BL8. Null mutation affecting the Sp7/MP2 serine protease that regulates melanization in Drosophila, isogenized in the w[1118] DrosDel genetic background. Sp7 strongly blocks the blackening reaction at the injury site of larvae but has less effect in adults (Dudzic et al., 2015).	Serine protease 7	DrosDel Immunity panel	Sign In to show prices
349909	349909	DD-Im	mutants	w[1118]; ; PGRP-SD[SK1]	BL10. Null mutation affecting the secreted pattern recognition receptor PGRP-SD that promotes the activation of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.	349909	Iatsenko et al., Immunity (2016)	Bruno Lemaitre with the help of Mark Hanson	EPFL	PGRP-SD	Dm PGRP-SD DrosDel Immunity panel PGRP-SD Peptidoglycan recognition protein SD Peptidoglycan-recognition protein-SD precursor pgrp-sd	BL10. Null mutation affecting the secreted pattern recognition receptor PGRP-SD that promotes the activation of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.	Peptidoglycan recognition protein SD	DrosDel Immunity panel	Sign In to show prices
349910	349910	DD-Im	mutants	w[1118]; ; PGRP-LB[Delta]	BL11. Null mutation affecting PGRP-LB (created by homologous replacement of PGRP-LB sequences with w[+]), which encodes a secreted negative regulator of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.	349910	Paredes et al., Immunity (2011)	Bruno Lemaitre with the help of Mark Hanson	EPFL	PGRP-LD	CG32912 CG5523 DrosDel Immunity panel PGRP-LD Peptidoglycan recognition protein LD Peptidoglycan recognition protein-LD	BL11. Null mutation affecting PGRP-LB (created by homologous replacement of PGRP-LB sequences with w[+]), which encodes a secreted negative regulator of the Imd pathway upstream of PGRP-LC, isogenized in the w[1118] DrosDel genetic background.	Peptidoglycan recognition protein LD	DrosDel Immunity panel	Sign In to show prices
349916	349916	DD-Im	mutants	w[1118]; ; Df(3R)CecA-C[Delta]	BL17. A deletion removing the Cecropin Locus (4 genes), all isogenized in the w[1118] DrosDel genetic background.	349916	Carboni et al., Genetics (2022)	Bruno Lemaitre with the help of Mark Hanson	EPFL	CecA1	CEC CG4740 Cec Cec A1 CecA CecA-C CecA1 CecA2 CecB CecC Cecropin Cecropin A Cecropin A1 Cecropin-A Cecropin-A1 Cecropin-A1/A2 precursor CecropinA CecropinA1 DrosDel Immunity panel cec cecA cec	BL17. A deletion removing the Cecropin Locus (4 genes), all isogenized in the w[1118] DrosDel genetic background.	Cecropin A1	DrosDel Immunity panel	Sign In to show prices
349917	349917	DD-Im	mutants	w[1118]; ; AttD[SK1]	BL18. A mutation affecting Attacin D, isogenized in the w[1118] DrosDel genetic background.	349917	Hanson et al., eLife 8 (2019)	Bruno Lemaitre with the help of Mark Hanson	EPFL	AttD	Att AttD Attacin Attacin D Attacin-D AttacinD Attackin DrosDel Immunity panel att att D attD attacin attacin D	BL18. A mutation affecting Attacin D, isogenized in the w[1118] DrosDel genetic background.	Attacin-D	DrosDel Immunity panel	Sign In to show prices
349924	349924	DD-Im	mutants	w[1118]; ; Drs[R1]	BL25. A deletion removing the Drs locus (generated by homologous recombination, contains a w+ gene), isogenized in the w[1118] DrosDel genetic background	349924	Hanson et al., eLife 8 (2019)	Bruno Lemaitre with the help of Mark Hanson	EPFL	Drs	BcDNA:LP03851 Crp Cysteine-rich-protein DIM 19 DIM 21 DRO DROM DROS DRS Drm Drom Dros DrosDel Immunity panel Droso Drosomcycin Drosomycin Drosomycin precursor Drosomycin-B Drosomycine Drosomyocin	BL25. A deletion removing the Drs locus (generated by homologous recombination, contains a w+ gene), isogenized in the w[1118] DrosDel genetic background	Drosomycin	DrosDel Immunity panel	Sign In to show prices
349930	349930	DD-Im	mutants	w[1118]; ; Df(3L)LysB-P[Delta]	BL31. An 11.5-kb deletion, removing LysC (a putative pseudogene) and the 4 lysozyme genes (i.e., Lys B, LysD, LysE, and Lys P) that are known to be strongly induced in the gut; isogenized in the w[1118] DrosDel genetic background.	349930	Marra et al., mBio (2021)	Bruno Lemaitre with the help of Mark Hanson	EPFL	LysB	DrosDel Immunity panel Lys B Lys P LysB LysC LysD LysE Lysozyme B Lyzozyme B lysB	BL31. An 11.5-kb deletion, removing LysC (a putative pseudogene) and the 4 lysozyme genes (i.e., Lys B, LysD, LysE, and Lys P) that are known to be strongly induced in the gut; isogenized in the w[1118] DrosDel genetic background.	Lysozyme B	DrosDel Immunity panel	Sign In to show prices
349934	349934	DD-Im	mutants	w[1118]; ; TotAZ[SK6]	BL35. A fly line, called TotAZ, with a deletion removing the TotA, TotB, TotC and TotZ genes, isogenized in the w[1118] DrosDel genetic background.	349934	Rommelaere et al., Curr Biol (2024)	Bruno Lemaitre with the help of Mark Hanson	EPFL	TotA	DrosDel Immunity panel Stress-inducible humoral factor Turandot A Tot Tot A Tot-A TotA TotAZ TotB TotC TotZ Turandot Turandot A Turt tot totA turandot A	BL35. A fly line, called TotAZ, with a deletion removing the TotA, TotB, TotC and TotZ genes, isogenized in the w[1118] DrosDel genetic background.	Turandot A	DrosDel Immunity panel	Sign In to show prices