302001
    
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VA-STOCK-302001
| VDRC Id | 302001 | 
|---|---|
| Library | VA-STOCK | 
| Sub-Category | RNAi, tagged construct | 
| Status | available | 
| Comment | Expresses dsRNA for RNAi of polo. Expresses GFP-tagged polo with a nonphosphorylatable T182A substitution under UASp control. Synonymous substitutions make polo sequence resistant to RNAi by P{GD7563}. Homozygotes present., | 
| Construct Id | 302001 | 
| Genotype | w[*]; P{w[+mC]=GD7563}v20177; P{y[+t7.7] w[+mC]=UASp-polo.T182A.GFP.RNAi-res}attP154/TM6B, Tb[+] | 
| Synonyms | RNAi, tagged construct polo  | 
                
| Keywords | RNAi, tagged construct polo  | 
                
| Sequence | empty | 
| Viability | unknown | 
| Publication | Kachaner et al. (2017). Coupling of Polo kinase activation to nuclear localization by a bifunctional NLS is required during mitotic entry. Nat. Commun. 8(1): 1701. |