111002
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SKU
KK-111002
| VDRC Id | 111002 |
|---|---|
| Library | KK |
| Status | available |
| Comment | KK RNAi line similar to VDRC ID 106174, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Construct Id | 101055 |
| CG Number (r6.01) | CG12072 |
| FlyBase gene number | FBgn0011739 FBgn0013549 |
| Synonyms | Dlats HD12aCFD0196 LATS LATS1 Lats Lats/Warts MENE (3R)-G WTS/LATS Warts Warts kinase Warts/Lats Wts Wts/Lats dmLATS l(3)100Aa large tumor suppressor large tumor supressor lats lts miracle-gro wa |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 379 |
| ON Targets | 1 |
| OFF Targets | 1 |
| CAN Repeats | 2 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCACCCAGTTATTGTCGGCTT |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgacgcccgctgataGTTCTTCATGGAGCAGCACA |
| Right Primer PCR2 | GCGCTCTAGACGACGCCCGCTGATA(XTS1) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | CACCCAGTTATTGTCGGCTTCCGCGAGGATATCCCTCTCGGCCTTCACGTGTGCCACCTGATTCCGCTTGAGAACGTCCGCTTTCCGCAGGGTTTTCATCGCATACAAATGGTTCGAGGTATCGATTTTGCTCACCAGCGTTACCTCGCCAAATGCACCCACTCCAATGGGCTTCAGTTTGACGAACATGCTCTTGTCCATCTTGGCGCGCTTCAATCGAATGTAGTTGCTCTCCTTTTGGTTCAGCATTTTCCTCATCTCGATTTGGGTCTGATCGGGCAGTCCCACTTTGTGCATCTCCTTCTCCAGCTGATTCTTGCGATACGTGCGCTGGCGATACGACTTGATCACGTTCTCTATGTGCTGCTCCATGAAGAAC |
| Publication | Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |
| Provider Scientist | Kieran Harvey |
| Provider Organization | Peter MacCallum Cancer Centre, Melbourne, Australia |