106053
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SKU
KK-106053
| VDRC Id | 106053 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 102518 |
| CG Number (r6.01) | CG9256 |
| FlyBase gene number | FBgn0032917 FBgn0032918 FBgn0040297 |
| Synonyms | CG9255 DmNHE2 HD12aCFD0142 NHE2 Na[+]/H[+] hydrogen exchanger 2 Nhe2 nhe2 |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 459 |
| ON Targets | 1 |
| OFF Targets | 1 |
| CAN Repeats | 2 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgCTGAAAGGAACCTGGCTGAG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgcatgtagcctgccCAATGTCCTGGCTGACCTTT |
| Right Primer PCR2 | GCGCTCTAGACGCATGTAGCCTGCC(XTS3) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | CTGAAAGGAACCTGGCTGAGTAGAAGTACGTTGTAGCATGCCAGGATCAAAAAAGTGAATAACAGATCATAATGAGCATCCGCACGGAGCAGGATTACGACAGTGCCACTCCGGCGCTGGCACAGCAAATGAATTTGGCCCGAAGAGCCTGCTGGAGGATCAAATCCTACAGCTCGGAATCCCTCTTCAAGACCTATGCTTCTGTAATAACAGATACTAGTGCAAATGAAATCGATGCAGAAGCACCTCCACCACCGAGAGATAAAACCAAAACGAGAATAGAGCCACAGATAGCGCCCGCAAAACGCAACTCATCCTGCACATCATCCGACTGGCGGGGGATGTTCAGCAAAAGGACGCTGCTCATCTGCGCCCTGGCACTGATCCTTGGAAGTGCCCAGGCGCGGCCCAACACGAGTGCTGTGGGAGTGGCTCCGAGAAAGGTCAGCCAGGACATTG |