100310
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SKU
KK-100310
| VDRC Id | 100310 |
|---|---|
| Library | KK |
| Status | available |
| Construct Id | 107917 |
| CG Number (r6.01) | CG9075 |
| FlyBase gene number | FBgn0001942 FBgn0013406 FBgn0013523 |
| Synonyms | 2439 9075 Dm-eIF4A DmRH2 EIF-4A EIF-4a EIF4A EiF4A Eif-4a Eif4a Eukaryotic initiation factor 4A Eukaryotic initiation factor 4a L(2L)162 Su(dpp)YE9 anon-26Aa anon26A deIF4A eIF 4A eIF-4A eIF-4a e |
| Inserted Chromosome | 2 |
| Viability | viable |
| Hairpin Length | 281 |
| ON Targets | 1 |
| OFF Targets | 1 |
| s19 | 0.99 |
| CAN Repeats | 2 |
| Insertion @ 40D | N |
| Insertion @ 30B | Y |
| Left Primer PCR1 | tggcgcccctagatgACATCAAGCTGTTCGTGCTG |
| Left Primer PCR2 | GCGCGAATTCTGGCGCCCCTAGATG(ETU) |
| Right Primer PCR1 | cgcatgtagcctgccATCGTACAGATCGCAAAGGG |
| Right Primer PCR2 | GCGCTCTAGACGCATGTAGCCTGCC(XTS3) |
| Sequence Verification Primer | ETU |
| Template | genomic |
| Sequence | ACATCAAGCTGTTCGTGCTGGATGAGGCCGATGAGATGTTGTCCCGCGGTTTCAAGGATCAGATCCAGGATGTCTTCAAGATGCTGCCCCCAGATGTGCAGGTCATCCTGCTGTCCGCCACCATGCCGCCGGATGTGCTCGAAGTGAGCCGTTGCTTCATGCGTGATCCCGTCAGCATCCTGGTTAAGAAGGAGGAACTGACCCTTGAGGGTATCAAGCAGTTTTACGTCAACGTGAAGCAGGAGAACTGGAAACTGGGCACCCTTTGCGATCTGTACGAT |